Publications using Biogro media:


For a complete list of publications, click HERE 




Papers on virus production from Biogro-Vero/MDCK/MRC-5/BHK SFM

Liu C-C, Lee S-C, Butler M, Wu S-C. (2008) High genetic stability of dengue virus propagated in MRC-5 cells as compared to the virus propagated in vero cells. PLoS ONE 3(3): e1810. PDF file

Chia-Chyi Liu, Wei-Cheng Lian, Michael Butler, Suh-Chin Wu. (2007). High immunogenic enterovirus 71 strain and Its production using serum-free microcarrier Vero cell culture. Vaccine. 25(1):19-24. PDF file

Wu S-C, Liu, C-C, Butler, M. and Lian, W-C. A comparison of virus propagation, inactivation, and immunogenicity of three enterovirus-71 strains produced in serum-free microcarrier Vero cell culture. Cell Culture Engineering X Conference, Whistler, Canada 2006. PDF file

Burgener, A., Coombs, K. and Butler, M. (2006). Intracellular ATP and total adenylate concentrations are critical predictors of reovirus productivity from Vero cells. Biotech Bioeng 94 (4): 667-679. PDF file

Wu SC, Liu CC, Lian W-C. 2004. Optimization of microcarrier cell culture process for the inactivated enterovirus type 71 vaccine development. Vaccine. 22(29-30):3858-64. PDF file

Wu SC, Huang GY. 2002 Stationary and microcarrier cell culture processes for propagating Japanese encephalitis virus. Biotechnol Prog. 18(1):124-8. PDF file

Burgener, A., Patrick, M., Coombs, K., Moffatt, D., Huzel, N. and Butler, M. 2001. “The modification of a serum-free media formulation for the production of reovirus and the growth of Vero, MRC-5, MDCK and BHK cells” In "Animal Cell Technology: From target to market" (Lindner-Olsson, E, Chatzissavidou, N. and Lullau, E. eds.) European Society for Animal Cell Technology 17, 200-203. PDF file

Butler, M., Burgener, A., Patrick, M., Berry,M., Moffatt, D., Huzel, N., Barnabé,N. and Coombs,K. 2000. Application of a serum-free medium for the growth of Vero cells and the production of reovirus Biotech. Progress 16: 854-858. PDF file

Berry, J.M., Barnabé, N., Coombs,K. M. and Butler, M. 1999. Production of reovirus type-1 and type-3 from Vero cells grown on solid and macroporous microcarriers. Biotech. Bioeng. 62: 12-19. PDF file

Christie, A. and Butler, M. 1999. The adaptation of BHK cells to a non-ammoniagenic glutamate-based culture medium. Biotech & Bioeng 64 (3): 298-309. PDF file


Papers using Biogro-CHO serum-free medium

Sunley, K., Tharmalingam,T. and Butler,M. (2008) CHO cells adapted to hypothermic growth produce high yields of recombinant beta-interferon. Biotech Progress 24(4): 898-906. PDF file

Tharmalingam, T.Sunley, K. and Butler,M. (2008) High yields of monomeric recombinant beta-interferon from macroporous microcarrier cultures under hypothermic conditions. Biotech Progress 24(4): 832-838. PDF file

Tharmalingam, T,. Ghebeh, H,. Wuerz, T and Butler,M. (2008) Pluronic enhances the robustness and reduces the cell attachment of mammalian cells. Molecular Biotechnology 39(2), 167-77. PDF file

Spearman, M., Rodriguez, J. Huzel, N., Sunley, K. and Butler, M. (2007) Effect of Culture Conditions on Glycosylation of Recombinant beta-Interferon in CHO Cells. In "Animal Cell Technology: From target to market" (Smith, R. ed.) European Society for Animal Cell Technology 17, 200-203.  PDF file

Restelli, V, Wang, M-D, Huzel, N, Ethier, M, Perreault, H and Butler, M. (2006). The effect of dissolved oxygen on the production and the glycosylation profile of recombinant human erythropoietin produced from CHO cells. Biotech Bioeng 94 (3): 481-494. PDF file

Rodriguez, J., Spearman, M., Huzel, N. and Butler, M. (2005) Enhanced production of monomeric interferon-ß by CHO cells through the control of culture conditions. Biotechnology Progress 21(1) 22-30. PDF file

Spearman, M., Rodriguez, J., Huzel, N. and Butler, M. (2004) Production and glycosylation of recombinant beta-interferon in suspension and Cytopore microcarrier cultures of CHO cells. Biotechnology Progress 21(1) 31-39. PDF file

Yang,M. and Butler,M. 2002. Effects of ammonia and glucosamine on the heterogeneity of erythropoietin glycoforms. Biotechnology Progress. 18: 129-138. PDF file

Wang M-D, Yang, M., Huzel, N. and Butler, M. 2002 Erythropoietin production from CHO cells grown by continuous culture in a fluidized-bed bioreactor Biotech Bioeng 77:194-203. PDF file

Yang,M. and Butler,M. 2000. The effect of ammonia on the glycosylation of human recombinant erythropoietin in culture Biotech. Progress 16: 751-759. PDF file

Yang,M. and Butler,M. 2000. Effects of ammonia on CHO cell growth, erythropoietin production and glycosylation. Biotech. Bioeng. 68: 370-380. PDF file

Yang,M. and Butler,M. 2000. Enhanced erythropoietin heterogeneity in a CHO culture is caused by proteolytic degradation and can be eliminated by a high glutamine level. Cytotechnology 34: 83-99. PDF file


Papers using Biogro-hybridoma media

Barnabé, N., Butler, M. and Hasinoff,B.B. 2001 “The effect of the catalytic topoisomerase II inhibitor dexrazane (ICRF-187) on CC9C10 hybridoma viability and productivity” Cytotechnology 37: 107-117. PDF file

Barnabé, N. and Butler, M. 2000 The effect of glucose and glutamine on the intracellular nucleotide pool and oxygen uptake rate of a murine hybridoma. Cytotechnology 34 (1-2): 47-57. PDF file

Kunkel, J.P., Jan, D.C.H., Butler,M. and Jamieson, J.C. 2000. Comparisons of the glycosylation of a monoclonal antibody produced under nominally-identical cell culture conditions in two different bioreactors. Biotechnol. Progress 16 (3), 462-470.PDF file

Butler M., Kunkel J, Jan D., Huzel N. and Jamieson J. (2000) The glycosylation of immunoglobulin (IgG) in bioreactors. Biotecnologia Aplicada , 17(3): 191. PDF file

Broadhurst, E.R. and Butler, M. 2000. The inhibitory effect of glutamate on the growth of a murine hybridoma is caused by competitive inhibition of the x-C transport system required for cystine utilization. Cytotechnology 32: 45-61. PDF file

Butler, M., Huzel, N., Barnabé, N., Gray, T. and Bajno, L. 1999. Linoleic acid improves the robustness of cells in agitated cultures. Cytotechnology 30 (1-3): 27-36. PDF file

Kunkel, J.P., Jan, D.C.H., Jamieson, J.C. and Butler, M. 1998. Dissolved oxygen concentration in serum-free continuous culture affects N-linked glycosylation of a monoclonal antibody. J. Biotechnol. 62 (1); 55-71. PDF file

Where to Find Us:

107-865 Waverley St
Winnipeg, MB

R3T 5P4


Phone: (204) 488-0298

Print Print | Sitemap